[HTML][HTML] Expression and Characterization of Recombinant Caveolin: PURIFICATION BY POLYHISTIDINE TAGGING AND CHOLESTEROL-DEPENDENT …
S Li, KS Song, MP Lisanti - Journal of Biological Chemistry, 1996 - ASBMB
Caveolin, a 22-24-kDa integral membrane protein, is a principal component of caveolar
membranes in vivo. Caveolin has been proposed to function as a scaffolding protein to
organize and concentrate signaling molecules within caveolae. Because of its unusual
membrane topology, both the N-and C-terminal domains of caveolin remain entirely
cytoplasmic and are not subject to luminal modifications that are accessible to other integral
membrane proteins. Under certain conditions, caveolin also exists in a soluble form as a …
membranes in vivo. Caveolin has been proposed to function as a scaffolding protein to
organize and concentrate signaling molecules within caveolae. Because of its unusual
membrane topology, both the N-and C-terminal domains of caveolin remain entirely
cytoplasmic and are not subject to luminal modifications that are accessible to other integral
membrane proteins. Under certain conditions, caveolin also exists in a soluble form as a …