[HTML][HTML] Glutamine synthetase in muscle is required for glutamine production during fasting and extrahepatic ammonia detoxification
Y He, TBM Hakvoort, SE Köhler, JLM Vermeulen… - Journal of biological …, 2010 - ASBMB
The main endogenous source of glutamine is de novo synthesis in striated muscle via the
enzyme glutamine synthetase (GS). The mice in which GS is selectively but completely
eliminated from striated muscle with the Cre-loxP strategy (GS-KO/M mice) are,
nevertheless, healthy and fertile. Compared with controls, the circulating concentration and
net production of glutamine across the hindquarter were not different in fed GS-KO/M mice.
Only a∼ 3-fold higher escape of ammonia revealed the absence of GS in muscle. However …
enzyme glutamine synthetase (GS). The mice in which GS is selectively but completely
eliminated from striated muscle with the Cre-loxP strategy (GS-KO/M mice) are,
nevertheless, healthy and fertile. Compared with controls, the circulating concentration and
net production of glutamine across the hindquarter were not different in fed GS-KO/M mice.
Only a∼ 3-fold higher escape of ammonia revealed the absence of GS in muscle. However …