[HTML][HTML] Activation-induced cytidine deaminase auto-activates and triggers aberrant gene expression
T Isobe, SNJ Song, P Tiwari, H Ito, Y Yamaguchi… - FEBS letters, 2013 - Elsevier
T Isobe, SNJ Song, P Tiwari, H Ito, Y Yamaguchi, K Yoshizaki
FEBS letters, 2013•ElsevierDNA methylation is a well-characterized epigenetic landmark involved in transcriptional
regulation; however, mechanisms underlying its regulation remain poorly characterized.
Recent studies demonstrate that activation-induced cytidine deaminase (AID) is involved in
active DNA demethylation. AID is aberrantly expressed in inflammation-associated cancers
and generates point mutations; however, cellular disorders attributed to its demethylation
function are largely unexplored. Here we demonstrate that ectopic AID expression perturbs …
regulation; however, mechanisms underlying its regulation remain poorly characterized.
Recent studies demonstrate that activation-induced cytidine deaminase (AID) is involved in
active DNA demethylation. AID is aberrantly expressed in inflammation-associated cancers
and generates point mutations; however, cellular disorders attributed to its demethylation
function are largely unexplored. Here we demonstrate that ectopic AID expression perturbs …
Abstract
DNA methylation is a well-characterized epigenetic landmark involved in transcriptional regulation; however, mechanisms underlying its regulation remain poorly characterized. Recent studies demonstrate that activation-induced cytidine deaminase (AID) is involved in active DNA demethylation. AID is aberrantly expressed in inflammation-associated cancers and generates point mutations; however, cellular disorders attributed to its demethylation function are largely unexplored. Here we demonstrate that ectopic AID expression perturbs tumor-related gene expression. AID (with Gadd45) activated a methylated paired box gene 5 (Pax5) reporter construct, and induced expression and association of endogenous Pax5 with the AID promoter, suggesting that aberrant AID expression triggers an auto-activation circuit to consolidate self-expression.
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