Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination
O Smithies, RG Gregg, SS Boggs, MA Koralewski… - Nature, 1985 - nature.com
O Smithies, RG Gregg, SS Boggs, MA Koralewski, RS Kucherlapati
Nature, 1985•nature.comA 'rescuable'plasmid containing globin gene sequences allowing recombination with
homologous chromosomal sequences has enabled us to produce, score and clone
mammalian cells with the plasmid integrated into the human β-globin locus. The planned
modification was achieved in about one per thousand transformed cells whether or not the
target gene was expressed.
homologous chromosomal sequences has enabled us to produce, score and clone
mammalian cells with the plasmid integrated into the human β-globin locus. The planned
modification was achieved in about one per thousand transformed cells whether or not the
target gene was expressed.
Abstract
A ‘rescuable’ plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human β-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
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