Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli
J Donaghy, PF Kelly, AM McKay - Applied Microbiology and Biotechnology, 1998 - Springer
J Donaghy, PF Kelly, AM McKay
Applied Microbiology and Biotechnology, 1998•SpringerThe production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected
in an agar-plate assay. The assay involves the substitution of the main carbon source in
specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains,
were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli,
Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The
enzyme was released from harvested cells by sonication and showed pH and temperature …
in an agar-plate assay. The assay involves the substitution of the main carbon source in
specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains,
were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli,
Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The
enzyme was released from harvested cells by sonication and showed pH and temperature …
Abstract
The production of feruloyl esterase activity by Bacillus spp. and lactobacilli can be detected in an agar-plate assay. The assay involves the substitution of the main carbon source in specific agar with ethyl ferulate. A number of Bacillus spp., predominantly B. subtilis strains, were found to exhibit feruloyl esterase activity by this method. Of the examined lactobacilli, Lb. fermentum (NCFB 1751) showed the highest level of ferulic acid esterase activity. The enzyme was released from harvested cells by sonication and showed pH and temperature optima of 6.5 and 30 °C respectively.
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