[HTML][HTML] Enzymatic properties of human cytosolic phospholipase A2γ
A Stewart, M Ghosh, DM Spencer, CC Leslie - Journal of Biological …, 2002 - ASBMB
The enzymatic properties of cytosolic phospholipase A 2 γ (cPLA 2 γ), an isoform of 85-kDa
group IV cPLA 2 α (cPLA 2 α) were studied in vitro and when the enzyme was expressed in
cells. cPLA 2 γ expressed in Sf9 cells is associated with membrane. Membranes isolated
from [3 H] arachidonic acid-labeled Sf9 cells expressing cPLA 2 γ, constitutively release [3
H] arachidonic acid. The membrane-associated activity is inhibited by the group IV PLA 2
inhibitor methylarachidonyl fluorophosphonate, but not effectively by the group VI PLA 2 …
group IV cPLA 2 α (cPLA 2 α) were studied in vitro and when the enzyme was expressed in
cells. cPLA 2 γ expressed in Sf9 cells is associated with membrane. Membranes isolated
from [3 H] arachidonic acid-labeled Sf9 cells expressing cPLA 2 γ, constitutively release [3
H] arachidonic acid. The membrane-associated activity is inhibited by the group IV PLA 2
inhibitor methylarachidonyl fluorophosphonate, but not effectively by the group VI PLA 2 …
