The human poly (A)-binding protein 1 shuttles between the nucleus and the cytoplasm
E Afonina, R Stauber, GN Pavlakis - Journal of Biological Chemistry, 1998 - ASBMB
We have studied the intracellular localization of poly (A)-binding protein 1 (PABP1) by
indirect immunofluorescence as well as by tagging with the green fluorescent protein (GFP)
in living cells. We show that PABP1 is able to enter the nucleus. Accumulation of PABP1 in
the nuclei was observed upon transcription inhibition, suggesting that active transcription is
required for PABP1 export. The nuclear import of PABP1 is an energy-dependent process
since PABP1 fails to enter the nucleus upon ATP depletion and at low temperature …
indirect immunofluorescence as well as by tagging with the green fluorescent protein (GFP)
in living cells. We show that PABP1 is able to enter the nucleus. Accumulation of PABP1 in
the nuclei was observed upon transcription inhibition, suggesting that active transcription is
required for PABP1 export. The nuclear import of PABP1 is an energy-dependent process
since PABP1 fails to enter the nucleus upon ATP depletion and at low temperature …